acid nucleic sythesis



The ordered assembly of deoxyribonucleotides into DNA and of ribonucleotides into RNA involves somewhat simpler cellular mechanisms than the correct assembly of the amino acids in a protein chain. Here we consider a few general principles governing the formation of polynucleotide chains in cells and briefly discuss
Weiter zu Synthesis of nucleic acids -
Nucleic acids are biopolymers, or small biomolecules, essential to all known forms of life. They are composed of nucleotides, which are monomers made of three components: a 5-carbon sugar, a phosphate group and a nitrogenous base. If the sugar is a simple ribose, the polymer is RNA (ribonucleic acid); if the sugar is
Nucleic acids, including deoxynucleic acid (DNA) and ribonucleic acid (RNA), store genetic information for living organisms. The production and regulation of these biological macromolecules are essential for survival and replication of organisms. Therefore, enzymes involved in these processes are attractive therapeutic
Nucleic Acids. 1. Introduction. The first isolation of what we now refer to as DNA was accomplished by Johann Friedrich Miescher circa 1870. He reported finding a weakly acidic substance of unknown function in the nuclei of human white blood cells, and named this material "nuclein". A few years later, Miescher separated
Nucleic acids are polymers of acidic monomeric subunits known as nucleotides. The nucleotides form a duplex, or double-stranded, molecule referred to as deoxyribonucleic acid (DNA) that stores genetic information within the cell. The genetic information in DNA is transferred to ribonucleic acid (RNA), monomeric forms of
Nucleic acid synthesis columns come in several scales, which are a function of the amount of the 3′ nucleoside coupled to the support material. Popular column sizes range from 40 nmol to 10 μmol. Popular support materials include controlled-pore glass (CPG) and polystyrene. The CPG columns are typically supplied
major problems to be posed were those of sequence and synthesis of poiy- nucleotides. In principle, organic chemists could attempt to put together nucleosides and nucleotides to form short chains of polynucleotides which would be identical in every way with the naturally-occurring nucleic acids. It was very reminiscent of
21.09.2011 -
11 Synthesis of Nucleic Acids. 11.1 Introduction. Advances in DNA synthesis, which have made it possible to widely use manmade DNAs in molecular biology, genetics, virology, and other fields, represent the most important contribution to the chemistry of nucleic acids in recent years. Along with achievements in molecular

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